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Rabbit anti-EPS15 IHC Antibody Affinity Purified

bethyl
Bethyl Laboratories

Catalog #

Validated
Target:

EPS15

Reactivity:

Human

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

Between 575 and 625

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (50+ slides)

10 µl (5+ slides)

Product Details

Specifications
Verified Reactivity

Human

Presumed Reactivity

Monkey

Antigen Species

Human

Concentration
100 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide


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Production Procedures

Antibody was affinity purified using an epitope specific to EPS15 immobilized on solid support.

The epitope recognized by IHC-00337 maps to a region between residue 575 and 625 of human epidermal growth factor receptor pathway substrate 15 using the numbering given in entry NP_001972.1 (GeneID 2060).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Antibody was affinity purified using an epitope specific to EPS15 immobilized on solid support.

The epitope recognized by IHC-00337-T maps to a region between residue 575 and 625 of human epidermal growth factor receptor pathway substrate 15 using the numbering given in entry NP_001972.1 (GeneID 2060).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Additional Product Information

EPS15 (epidermal growth factor receptor substrate 15) is a protein phosphorylated by the EGF receptor and involved in the internalization of the EGF-receptor via clathrin-coated pits.

2060

EPS15

epidermal growth factor receptor pathway substrate 15

P42566

Epidermal growth factor receptor substrate 15

Alternate Names

AF1P; AF-1P; ALL1 fused gene from chromosome 1; epidermal growth factor receptor substrate 15; MLLT5; protein AF-1p; Protein Eps15

Applications

Epitope exposure is recommended.

Epitope exposure with citrate buffer will enhance staining.

Likely to work with frozen sections.

In some cases, the antibody may be diluted further than indicated.