Bethyl Laboratories

Rabbit anti-EPS15 IHC Antibody Affinity Purified

Primary
Catalog #IHC-00337
TargetEPS15
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC, IHC-IF
Detection of human EPS15 by immunohistochemistry.
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Product Description

EPS15 (epidermal growth factor receptor substrate 15) is a protein phosphorylated by the EGF receptor and involved in the internalization of the EGF-receptor via clathrin-coated pits.

Specifications

Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 575 and 625
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
100 µg/ml
Target Identity
TargetEPS15
Antigen Species
Human
Gene ID2060
Gene Symbol
EPS15
Gene Name
epidermal growth factor receptor pathway substrate 15
Uniprot IDP42566
Protein Name
Epidermal growth factor receptor substrate 15
Alternate Names
Product Information
Applications
IHC, IHC-IF
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA

Production & Epitope

Antibody was affinity purified using an epitope specific to EPS15 immobilized on solid support.

The epitope recognized by IHC-00337 maps to a region between residue 575 and 625 of human epidermal growth factor receptor pathway substrate 15 using the numbering given in entry NP_001972.1 (GeneID 2060).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Applications

Epitope exposure is recommended.

Epitope exposure with citrate buffer will enhance staining.

Likely to work with frozen sections.

In some cases, the antibody may be diluted further than indicated.

Immunohistochemistry (IHC)1:100 - 1:500
Immunohistochemistry-Immunofluoresence (IHC-IF)1:50 - 1:500

Documents

Safety Data SheetPDF · IHC-00337Open
Product DatasheetPDF · IHC-00337 · Lot 1 (current lot)Open
Product DatasheetPDF · IHC-00337 · Lot 1 (current lot)Open
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