Bethyl Laboratories
Rabbit anti-ERCC3 Antibody Affinity Purified
Primary
Catalog #A301-336A
TargetERCC3
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC, IP
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Product Description
ERCC3 is a component of the core TFIIH basal transcription factor and functions as an ATP-dependent 3’-5’ DNA helicase. ERCC3 is involved in nucleotide excision repair and is the cause of xeroderma pigmentosum complementation group B (XPB), also known as xeroderma pigmentosum II (XP2). Additionally, it has been found to be the cuase of Cockayne syndrome, and trichothiodystrophy (TTD). XPB is an autosomal recessive disease characterized by skin photosensitivity and a predisposition to skin cancer, and neurological abnormalities. Cockayne syndrome and TTD are similarly characterized by photosensitivity and neural abnormalities.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 575 and 625
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetERCC3
Antigen Species
Human
Gene ID2071
Gene Symbol
ERCC3
Gene Name
ERCC excision repair 3, TFIIH core complex helicase subunit
Uniprot IDP19447
Protein Name
TFIIH basal transcription factor complex helicase XPB subunit
Alternate Names
basic transcription factor 2 89 kDa subunit • BTF2 • BTF2 p89 • DNA excision repair protein ERCC-3 • DNA repair helicase • DNA repair protein complementing XP-B cells • excision repair cross-complementation group 3 • excision repair cross-complementing rodent repair deficiency, complementation group 3 • general transcription and DNA repair factor IIH helicase subunit XPB • GTF2H • RAD25 • TFIIH • TFIIH 89 kDa subunit • TFIIH basal transcription factor complex 89 kDa subunit • TFIIH basal transcription factor complex helicase XPB subunit • TFIIH p89 • TFIIH subunit XPB • TTD2 • xeroderma pigmentosum group B-complementing protein • xeroderma pigmentosum, complementation group B • XPB
Product Information
Applications
IHC, IP
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to ERCC3 immobilized on solid support.
The epitope recognized by A301-336A maps to a region between residue 575 and 625 of human excision repair cross-complementing rodent repair deficiency, complementation group 3 using the numbering given in entry NP_000113.1 (GeneID 2071).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:2000 to 1:10,000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Documents
Product DatasheetPDF · A301-336A · Lot 2 (current lot)Open
Product DatasheetPDF · A301-336A · Lot 2 (current lot)Open
Safety Data SheetPDF · A301-336AOpen
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