Bethyl Laboratories
Rabbit anti-ERCC5/XPG Antibody Affinity Purified
Primary
Catalog #A301-484A
TargetERCC5/XPG
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC, IP, WB
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Product Description
ERCC5 is a single-stranded DNA endonuclease involved in DNA excision repair. ERCC5 is an important component of the nucleotide excision repair (NER) pathway which is critical to the repair of DNA lesions caused by exposure to UV light. Defects in ERCC5 are the cause of xeroderma pigmentosum, an autosomal recessive disease characterized by a hypersensitivity to sunlight and a high predisposition to cancer on UV-exposed areas.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 650 and 700
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetERCC5/XPG
Antigen Species
Human
Gene ID2073
Gene Symbol
ERCC5
Gene Name
ERCC excision repair 5, endonuclease
Uniprot IDP28715
Protein Name
DNA repair protein complementing XP-G cells
Alternate Names
COFS3 • DNA excision repair protein ERCC-5 • DNA repair protein complementing XP-G cells • ERCC5-201 • ERCM2 • excision repair cross-complementation group 5 • excision repair cross-complementing rodent repair deficiency, complementation group 5 • UVDR • Xeroderma pigmentosum group G-complementing protein • xeroderma pigmentosum, complementation group G • XPG • XPGC • XPG-complementing protein
Product Information
Applications
IHC, IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to ERCC5/XPG immobilized on solid support.
The epitope recognized by A301-484A maps to a region between residue 650 and 700 of human excision repair cross-complementing rodent repair deficiency, complementation group 5 (xeroderma pigmentosum, complementation group G (Cockayne syndrome)) using the numbering given in entry NP_000114.2 (GeneID 2073).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:500 to 1:2000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A301-484A · Lot 4 (current lot)Open
Product DatasheetPDF · A301-484A · Lot 4 (current lot)Open
Safety Data SheetPDF · A301-484AOpen
Citations / Publications
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