Bethyl Laboratories
Rabbit anti-ERF Antibody Affinity Purified
Primary
Catalog #A301-702A
TargetERF
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIHC, IP
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Product Description
Ets2 repressor factor (ERF) is a member of the ETS (e26 transformation specific sequence) family of proteins. ETS family members bear an ETS-domain, a DNA binding domain that recognizes purine-rich sequences. ERF functions as a transcriptional repressor and is an effector of the Erk signaling pathway.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 498 and C-terminus
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetERF
Antigen Species
Human
Gene ID2077
Gene Symbol
ERF
Gene Name
ETS2 repressor factor
Uniprot IDP50548
Protein Name
ETS domain-containing transcription factor ERF
Alternate Names
Product Information
Applications
IHC, IP
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to ERF immobilized on solid support.
The epitope recognized by A301-702A maps to a region between residue 498 and 548 of human Ets2 repressor factor using the numbering given in entry NP_006485.2 (GeneID 2077).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:1000 - 1:5000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Documents
Product DatasheetPDF · A301-702A · Lot 1 (current lot)Open
Product DatasheetPDF · A301-702A · Lot 1 (current lot)Open
Safety Data SheetPDF · A301-702AOpen
Citations / Publications
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