Bethyl Laboratories
Rabbit anti-ERF Antibody Affinity Purified
Primary
Catalog #A301-702A
TargetERF
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIHC, IP
Image 1 of 2
local_shipping
Order within 8 hours and 30 minutes for shipment by today
Product Description
Ets2 repressor factor (ERF) is a member of the ETS (e26 transformation specific sequence) family of proteins. ETS family members bear an ETS-domain, a DNA binding domain that recognizes purine-rich sequences. ERF functions as a transcriptional repressor and is an effector of the Erk signaling pathway.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 498 and C-terminus
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetERF
Antigen Species
Human
Gene ID2077
Gene Symbol
ERF
Gene Name
ETS2 repressor factor
Uniprot IDP50548
Protein Name
ETS domain-containing transcription factor ERF
Alternate Names
Product Information
Applications
IHC, IP
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to ERF immobilized on solid support.
The epitope recognized by A301-702A maps to a region between residue 498 and 548 of human Ets2 repressor factor using the numbering given in entry NP_006485.2 (GeneID 2077).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:1000 - 1:5000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Documents
Product DatasheetPDF · A301-702A · Lot 1 (current lot)Open
Product DatasheetPDF · A301-702A · Lot 1 (current lot)Open
Safety Data SheetPDF · A301-702AOpen
Citations / Publications
Services
Antibody Conjugation
Antibody and conjugate formats developed together, built to your specification.
Request CustomizationCustom Monoclonal Antibody Discovery Services
Full custom monoclonal development, from immunization through validation.
Explore Monoclonal DevelopmentVHH Discovery
Single-domain antibodies for structurally difficult targets a conventional monoclonal can't reach.
Explore VHH DiscoveryResources
1