Bethyl Laboratories

Rabbit anti-ERF IHC Antibody Affinity Purified

Primary
Catalog #IHC-00392
TargetERF
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIHC
Detection of human ERF by immunohistochemistry.
Image 1 of 2
local_shipping

Order within 6 hours and 42 minutes for shipment by today

Product Description

Ets2 repressor factor (ERF) is a member of the ETS (e26 transformation specific sequence) family of proteins. ETS family members bear an ETS-domain, a DNA binding domain that recognizes purine-rich sequences. ERF functions as a transcriptional repressor and is an effector of the Erk signaling pathway.

Specifications

Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 498 and C-terminus
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
100 µg/ml
Target Identity
TargetERF
Antigen Species
Human
Gene ID2077
Gene Symbol
ERF
Gene Name
ETS2 repressor factor
Uniprot IDP50548
Protein Name
ETS domain-containing transcription factor ERF
Alternate Names
Product Information
Applications
IHC
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA

Production & Epitope

Antibody was affinity purified using an epitope specific to ERF immobilized on solid support.

The epitope recognized by IHC-00392 maps to a region between residue 498 and 548 of human Ets2 repressor factor using the numbering given in entry NP_006485.2 (GeneID 2077).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Applications

Epitope exposure is recommended.

Epitope exposure with citrate buffer will enhance staining.

Likely to work with frozen sections.

In some cases, the antibody may be diluted further than indicated.

Immunohistochemistry (IHC)1:100 - 1:500

Documents

Safety Data SheetPDF · IHC-00392Open
Product DatasheetPDF · IHC-00392 · Lot 1 (current lot)Open
Product DatasheetPDF · IHC-00392 · Lot 1 (current lot)Open
Using this product in your research?Published applications help other scientists evaluate reagents and accelerate assay development.Upload Your Publication

Services

Resources