Rabbit anti-ETFB Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A305-483A-T maps to a region between residue 125 to 175 of human Electron transfer flavoprotein subunit beta using the numbering given in entry P38117.3 (GeneID 2109).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to ETFB immobilized on solid support.
The epitope recognized by A305-483A maps to a region between residue 125 to 175 of human Electron transfer flavoprotein subunit beta using the numbering given in entry P38117.3 (GeneID 2109).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
ETFB is the electron-transfer-flavoprotein, beta polypeptide, which shuttles electrons between primary flavoprotein dehydrogenases involved in mitochondrial fatty acid and amino acid catabolism and the membrane-bound electron transfer flavoprotein ubiquinone oxidoreductase [taken from NCBI Entrez Gene (Gene ID: 2109)].
Alternate Names
beta-ETF; electron transfer flavoprotein beta subunit; electron transfer flavoprotein subunit beta; electron transfer flavoprotein, beta polypeptide; electron-transferring-flavoprotein, beta polypeptide; FP585; MADD
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.