Rabbit anti-ETFB Antibody Affinity Purified

Specifications

Production & Epitope

Antibody was affinity purified using an epitope specific to ETFB immobilized on solid support.

The epitope recognized by A305-484A maps to a region between residue 205 to 255 of human Electron transfer flavoprotein subunit beta using the numbering given in entry P38117.3 (GeneID 2109).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).

Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.