/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-Exonuclease 1 Antibody
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Primary Antibodies  

Rabbit anti-Exonuclease 1 Antibody Affinity Purified

bethyl
Bethyl Laboratories

Catalog #

Validated
Citations (15)
Target:

Exonuclease 1

Reactivity:

Human

Applications:

IP

,

WB

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

between 796 and 846

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (200 µg/ml)

10 µl (200 µg/ml)

Product Details

Specifications
Verified Reactivity

Human

Antigen Species

Human

Concentration
200 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide


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Additional Product Information

Exonuclease 1 is a 5'-3' double-stranded DNA exonuclease which may also possess a cryptic 3'-5' double-stranded DNA exonuclease activity. Exonuclease 1 functions in DNA mismatch repair (MMR) to excise mismatch-containing DNA tracts directed by strand breaks located either 5' or 3' to the mismatch. It also exhibits endonuclease activity against 5'-overhanging flap structures similar to those generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. Exonuclease 1 is required for somatic hypermutation (SHM) and class switch recombination (CSR) of immunoglobulin genes and is essential for male and female meiosis [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q9UQ84].

9156

EXO1

exonuclease 1

Q9UQ84

Exonuclease 1

Alternate Names

exonuclease 1; Exonuclease I; HEX1; hExo1; hExoI; rad2 nuclease family member, homolog of S. cerevisiae exonuclease 1

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.