Bethyl Laboratories
Rabbit anti-EYA3 Antibody Affinity Purified
Primary
Catalog #A302-690A
TargetEYA3
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP
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Product Description
EYA3 (eyes absent homolog 3) functions as a tyrosine phosphatase that specifically dephosphorylates 'Tyr-142' of histone H2AX (H2AXY142ph). EYA3 promotes efficient DNA repair by dephosphorylating H2AX and promoting the recruitment of DNA repair complexes containing MDC1. EYA3 coactivates SIX1. Together with SIX1 and DACH2 EYA3 seems to be involved in myogenesis. EYA3 may be involved in development of the eye [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q99504].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 1 and 50
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetEYA3
Antigen Species
Human
Gene ID2140
Gene Symbol
EYA3
Gene Name
EYA transcriptional coactivator and phosphatase 3
Uniprot IDQ99504
Protein Name
Eyes absent homolog 3
Alternate Names
Product Information
Applications
IP
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to EYA3 immobilized on solid support.
The epitope recognized by A302-690A maps to a region between residue 30 and 60 of human eyes absent homolog 3 using the numbering given in entry NP_001981.2 (GeneID 2140).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Documents
Product DatasheetPDF · A302-690A · Lot 1 (current lot)Open
Product DatasheetPDF · A302-690A · Lot 1 (current lot)Open
Safety Data SheetPDF · A302-690AOpen
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