Rabbit anti-FAF1 Antibody Affinity Purified

Product Details
Specifications
The epitope recognized by A302-810A maps to a region between residue 25 and 75 of human Fas (TNFRSF6) Associated Factor 1 using the numbering given in entry NP_008982.1 (GeneID 11124).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to FAF1 immobilized on solid support.
The epitope recognized by A302-810A-T maps to a region between residue 25 and 75 of human Fas (TNFRSF6) Associated Factor 1 using the numbering given in entry NP_008982.1 (GeneID 11124).
Additional Product Information
FAS-associated factor 1 (FAF1) binds to FAS antigen and can initiate apoptosis or enhance apoptosis initiated through FAS antigen. Initiation of apoptosis FAF1 requires a ubiquitin-like domain but not the FAS-binding domain [taken from NCBI Entrez Gene (Gene ID: 11124)].
Alternate Names
CGI-03; Fas (TNFRSF6) associated factor 1; FAS-associated factor 1; hFAF1; HFAF1s; TNFRSF6-associated factor 1; UBX domain protein 3A; UBX domain-containing protein 12; UBX domain-containing protein 3A; UBXD12; UBXN3A
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.