Bethyl Laboratories
Rabbit anti-FBW7 IHC Antibody Affinity Purified
Primary
Catalog #IHC-00348
TargetFBW7
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC
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Product Description
FBW7 (F-box and WD repeat domain containing 7) is a member of the F-box family of proteins. The F-box is a protein-protein interaction motif first identified in cyclin F. F-box proteins associate with SCFs (Skp1-cullin-F-box protein ligase complexes) which are E3 ligases that ubiquitinate proteins and target them for proteasomal degradation. FBW7 has been found to bind and specifically target cyclin E and the Notch intracellular domain (NICD) for ubiquitination. Defects in FBW7 may contribute to tumorigenesis.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 50 and 100
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
100 µg/ml
Target Identity
TargetFBW7
Antigen Species
Human
Gene ID55294
Gene Symbol
FBXW7
Gene Name
F-box and WD repeat domain containing 7
Uniprot IDQ969H0
Protein Name
F-box/WD repeat-containing protein 7
Alternate Names
AGO • archipelago • Archipelago homolog • CDC4 • F-box and WD repeat domain containing 7, E3 ubiquitin protein ligase • F-box and WD-40 domain protein 7 (archipelago homolog, Drosophila) • F-box and WD-40 domain-containing protein 7 • F-box protein FBW7 • F-box protein FBX30 • F-box protein SEL-10 • F-box/WD repeat-containing protein 7 • FBW6 • FBW7 • FBX30 • FBXO30 • FBXW6 • hAgo • hCdc4 • homolog of C elegans sel-10 • SEL10 • SEL-10
Product Information
Applications
IHC
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to FBW7 immobilized on solid support.
The epitope recognized by IHC-00348 maps to a region between residue 50 and 100 of human F-box and WD-40 domain protein 7 using the numbering given in entry NP_361014.1 (GeneID 55294).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
Epitope exposure is recommended.
Epitope exposure with citrate buffer will enhance staining.
Likely to work with frozen sections.
In some cases, the antibody may be diluted further than indicated.
Immunohistochemistry (IHC)1:100 - 1:500
Documents
Safety Data SheetPDF · IHC-00348Open
Product DatasheetPDF · IHC-00348 · Lot 1 (current lot)Open
Product DatasheetPDF · IHC-00348 · Lot 1 (current lot)Open
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