Bethyl Laboratories
Rabbit anti-FBXO38 Antibody Affinity Purified
Primary
Catalog #A302-378A
TargetFBXO38
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIHC, IP, WB
Image 1 of 2
local_shipping
Order within 13 hours and 26 minutes for shipment by Tuesday, November 17
Product Description
FBXO38 (F-box only protein 38) is a member of the the F-box protein family. Members of this family are characterized by an approximately 40-amino acid F-box motif. FBXO38 is also known as MoKA (modulator of KLF7 activity) and originally identified as a factor that super-stimulated the activity of the Kruppel-like zinc-finger protein, KLF7, which plays a role in the gene expression of genes encoding cell cycle regulators such as p21 Waf/Cip and p27 kip.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 850 and 900
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetFBXO38
Antigen Species
Human
Gene ID81545
Gene Symbol
FBXO38
Gene Name
F-box protein 38
Uniprot IDQ6PIJ6
Protein Name
F-box only protein 38
Alternate Names
Product Information
Applications
IHC, IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to FBXO38 immobilized on solid support.
The epitope recognized by A302-378A maps to a region between residue 825 and 875 of human F-box only protein 38 using the numbering given in entry NP_995308.1 (GeneID 81545).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:500 - 1:2000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A302-378A · Lot 1 (current lot)Open
Product DatasheetPDF · A302-378A · Lot 1 (current lot)Open
Safety Data SheetPDF · A302-378AOpen
Citations / Publications
Services
Antibody Conjugation
Antibody and conjugate formats developed together, built to your specification.
Request CustomizationCustom Monoclonal Antibody Discovery Services
Full custom monoclonal development, from immunization through validation.
Explore Monoclonal DevelopmentVHH Discovery
Single-domain antibodies for structurally difficult targets a conventional monoclonal can't reach.
Explore VHH DiscoveryResources
1