Bethyl Laboratories
Rabbit anti-Fen1 IHC Antibody Affinity Purified
Primary
Catalog #IHC-00335
TargetFen1
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC
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Product Description
Flap endonuclease 1 (Fen1) is a member of the XPG/RAD2 endonuclease family. Fen1 functions primarily as an endonuclease that removes 5’-overhangs during base-excision DNA repair and lagging strand DNA synthesis. For these processes Fen1 interacts with PCNA and the 9-1-1 DNA repair complex. Fen1 also possesses 5’ to 3’ exonuclease activity as well as RNase H activity.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 250 and 300
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
500 µg/ml
Target Identity
TargetFen1
Antigen Species
Human
Gene ID2237
Gene Symbol
FEN1
Gene Name
flap structure-specific endonuclease 1
Uniprot IDP39748
Protein Name
Flap endonuclease 1
Alternate Names
Product Information
Applications
IHC
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to Fen1 immobilized on solid support.
The epitope recognized by IHC-00335 maps to a region between residues 250 and 300 of human Flap Structure Endonuclease 1 using the numbering given in entry NP_004102.1 (GeneID 2237).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
Epitope exposure is recommended.
Epitope exposure with citrate buffer will enhance staining.
Overnight incubation at room temperature is recommended.
Likely to work with frozen sections.
Immunohistochemistry (IHC)1:100 - 1:500
Documents
Safety Data SheetPDF · IHC-00335Open
Product DatasheetPDF · IHC-00335 · Lot 1 (current lot)Open
Product DatasheetPDF · IHC-00335 · Lot 1 (current lot)Open
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