Bethyl Laboratories
Rabbit anti-FHOD1 Antibody Affinity Purified
Primary
Catalog #A304-824A
TargetFHOD1
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP, WB
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Product Description
FHOD1 is required for the assembly of F-actin structures, such as stress fibers. It depends on the Rho-ROCK cascade for its activity. FHOD1 contributes to the coordination of microtubules with actin fibers and plays a role in cell elongation. It acts synergistically with ROCK1 to promote SRC-dependent non-apoptotic plasma membrane blebbing [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q9Y613].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 1050 and 1100
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetFHOD1
Antigen Species
Human
Gene ID29109
Gene Symbol
FHOD1
Gene Name
formin homology 2 domain containing 1
Uniprot IDQ9Y613
Protein Name
FH1/FH2 domain-containing protein 1
Product Information
Applications
IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to FHOD1 immobilized on solid support.
The epitope recognized by A304-824A maps to a region between residue 1050 to 1100 of human FH1/FH2 domain-containing protein 1 using the numbering given in entry Q9Y613.3 (GeneID 29109).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A304-824A · Lot 1 (current lot)Open
Product DatasheetPDF · A304-824A · Lot 1 (current lot)Open
Safety Data SheetPDF · A304-824AOpen
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