Bethyl Laboratories
Rabbit anti-Fibroblast Activation Protein alpha/FAP Recombinant Monoclonal Antibody [BLR150J]
Monoclonal Antibodies
Catalog #A700-150
TargetFibroblast Activation Protein alpha/FAP
HostRabbit
CloneBLR150J
ReactivityHuman
ApplicationICC, IHC, IP, WB
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Product Description
Fibroblast Activation Protein alpha, or FAP, is a homodimeric integral membrane gelatinase belonging to the serine protease family. It is selectively expressed in reactive stromal fibroblasts of epithelial cancers, granulation tissue of healing wounds, and malignant cells of bone and soft tissue sarcomas. FAP is involved in the control of fibroblast growth or epithelial-mesenchymal interactions during development, tissue repair, and epithelial carcinogenesis. [taken from NCBI Entrez Gene (Gene ID: 2191)].
Specifications
Clonality
Recombinant Monoclonal
Clone
BLR150J
Host
Rabbit
Immunogen
Residues 26-760 (ECD)
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Purified
Concentration
250 µg/ml
Target Identity
Antigen Species
Human
Gene ID2191
Gene Symbol
FAP
Gene Name
fibroblast activation protein alpha
Uniprot IDQ12884
Protein Name
Prolyl endopeptidase FAP
Alternate Names
170 kDa melanoma membrane-bound gelatinase • dipeptidyl peptidase FAP • DPPIV • FAPA • FAPalpha • Fibroblast activation protein alpha • gelatine degradation protease FAP • integral membrane serine protease • post-proline cleaving enzyme • prolyl endopeptidase FAP • seprase • Serine integral membrane protease • SIMP • surface-expressed protease
Product Information
Applications
ICC, IHC, IP, WB
Buffer
Phosphate Buffered Saline (PBS) with 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Recombinant antibody was purified from cell culture supernatant.
Immunogen was a recombinant protein representing the extracellular domain of human Prolyl Endopeptidase FAP (residues 26-760, NP_004451.2).
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunocytochemistry (ICC)1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
Immunohistochemistry (IHC)1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)20 µl/mg lysate
Western Blot (WB)1:1000
Documents
Product DatasheetPDF · A700-150 · Lot 3 (current lot)Open
Product DatasheetPDF · A700-150 · Lot 3 (current lot)Open
Product DatasheetPDF · A700-150 · Lot 201021AOpen
Safety Data SheetPDF · A700-150Open
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