Bethyl Laboratories
Rabbit anti-Filamin A Antibody Affinity Purified
Primary
Catalog #A301-133A
TargetFilamin A
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC, WB
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Product Description
Filamin A is an actin-binding protein that crosslinks and promotes orthogonal branching of actin filaments and also functions to anchor transmembrane proteins and intracellular signaling proteins to the actin cytoskeleton. As a scaffolding protein, filamin A is able to act as a link between cell membrane receptors and intracellular signaling proteins. Defects in filamin A are associated with multiple human diseases characterized by abnormalities in bone and neurological development.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 400 and 450
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetFilamin A
Antigen Species
Human
Gene ID2316
Gene Symbol
FLNA
Gene Name
filamin A
Uniprot IDP21333
Protein Name
Filamin-A
Alternate Names
ABP-280 • ABPX • actin binding protein 280 • Actin-binding protein 280 • alpha-filamin • CSBS • CVD1 • endothelial actin-binding protein • epididymis secretory sperm binding protein • FGS2 • filamin A, alpha • filamin-1 • filamin-A • FLN • FLN1 • FLN-A • FMD • MNS • NHBP • non-muscle filamin • OPD • OPD1 • OPD2 • XLVD • XMVD
Product Information
Applications
IHC, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to Filamin A immobilized on solid support.
The epitope recognized by A301-133A maps to a region between residue 400 and 450 of human Filamin A using the numbering given in entry NP_001447.1 (GeneID 2316).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Immunohistochemistry (IHC)1:500 - 1:2000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Western Blot (WB)1:2000 - 1:10,000 (3-8% Tris-acetate gel recommended)
Documents
Product DatasheetPDF · A301-133A · Lot 1 (current lot)Open
Product DatasheetPDF · A301-133A · Lot 1 (current lot)Open
Safety Data SheetPDF · A301-133AOpen
Citations / Publications
Services
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