Rabbit anti-FITC Recombinant Monoclonal Antibody [BLR304M]
Product Details
Specifications
Request Formulation Change Phosphate Buffered Saline (PBS) containing 0.09% Sodium Azide, BSA-Free
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Immunogen was Fluorescein isothiocyanate (FITC)-conjugated Keyhole Limpet Hemocyanin (KLH).
Additional Product Information
Fluorescein isothiocyanate (FITC) is a fluorescent conjugate that is used to label amine-containing molecules like proteins and peptides. This molecule has an excitation wavelength of 491 nm and an emission wavelength of 516 nm.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.