Bethyl Laboratories
Rabbit anti-FKBP5/FKBP51 Antibody Affinity Purified
Primary
Catalog #A301-429A
TargetFKBP5/FKBP51
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP
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Product Description
FKBP5/FKBP51 is an immunophilin and target of FK506, a macrolide immunosuppressive drug derived from Streptomyces tsukubaensis. Like other FK506 binding proteins, FKBP5/FKBP51 exhibits peptidyl-prolyl cis-trans isomerase activity. The most studied role of FKBP5/FKBP51 surrounds its involvement in the regulation of steroid receptor signaling. In association with the molecular chaperones Hsp90 and Hsp70, FKBP5/FKBP51 is able to influence steroid-hormone binding affinity and transcriptional activity. Recently, FKBP5/FKBP51 has become of interest in the field of psychiatry due to its potential role in cortisol regulation and its relation to mood disorders.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 225 and 275
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetFKBP5/FKBP51
Antigen Species
Human
Gene ID2289
Gene Symbol
FKBP5
Gene Name
FK506 binding protein 5
Uniprot IDQ13451
Protein Name
Peptidyl-prolyl cis-trans isomerase FKBP5
Alternate Names
51 kDa FK506-binding protein • 51 kDa FKBP • 54 kDa progesterone receptor-associated immunophilin • AIG6 • androgen-regulated protein 6 • FF1 antigen • FK506-binding protein 5 • FKBP-5 • FKBP51 • FKBP-51 • FKBP54 • HSP90-binding immunophilin • P54 • peptidylprolyl cis-trans isomerase • peptidyl-prolyl cis-trans isomerase FKBP5 • PPIase • PPIase FKBP5 • Ptg-10 • rotamase • T-cell FK506-binding protein
Product Information
Applications
IP
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to FKBP5/FKBP51 immobilized on solid support.
The epitope recognized by A301-429A maps to a region between residue 225 and 275 of human FK506 binding protein 5 (51 kDa FK506-binding protein) using the numbering given in entry NP_004108.1 (GeneID 2289).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Documents
Product DatasheetPDF · A301-429A · Lot 1 (current lot)Open
Product DatasheetPDF · A301-429A · Lot 1 (current lot)Open
Safety Data SheetPDF · A301-429AOpen
Citations / Publications
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