Bethyl Laboratories
Rabbit anti-FOXM1 Antibody Affinity Purified
Primary
Catalog #A301-533A
TargetFOXM1
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP, WB
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Product Description
FOXM1 belongs to a large family of Forkhead transcription factors that possesses a winged-helix DNA-binding domain called Forkhead box (FOX). It is a transcription factor critical to cell cycle progression via its role in coordinating the G2/M phase transition. FOXM1 is ubiquitously expressed in proliferating cells but is absent in differentiated cells. FOXM1 overexpression has been detected in a variety of tumor types including breast, lung, prostate, and liver cancer, and appears to be involved in tumorigenesis-associated processes such as angiogenesis and metastasis. In light of these findings, FOXM1 has recently become an attractive target for anti-cancer therapy.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 713 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetFOXM1
Antigen Species
Human
Gene ID2305
Gene Symbol
FOXM1
Gene Name
forkhead box M1
Uniprot IDQ08050
Protein Name
Forkhead box protein M1
Alternate Names
a novel forkhead box M1 isoform FOXM1D • FKHL16 • forkhead box protein M1 • Forkhead, drosophila, homolog-like 16 • forkhead-related protein FKHL16 • FOXM1A • FOXM1B • FOXM1C • hepatocyte nuclear factor 3 forkhead homolog 11 • HFH11 • HFH-11 • HNF-3 • HNF-3/fork-head homolog 11 • INS-1 • M-phase phosphoprotein 2 • MPHOSPH2 • MPM-2 reactive phosphoprotein 2 • MPP2 • MPP-2 • PIG29 • transcription factor Trident • TRIDENT • winged-helix factor from INS-1 cells
Product Information
Applications
IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to FOXM1 immobilized on solid support.
The epitope recognized by A301-533A maps to a region between residue 713 and 763 of human forkhead box M1 using the numbering given in entry NP_973731.1 (GeneID 2305).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:1000 - 1:5000
Documents
Product DatasheetPDF · A301-533A · Lot 2 (current lot)Open
Product DatasheetPDF · A301-533A · Lot 2 (current lot)Open
Safety Data SheetPDF · A301-533AOpen
Citations / Publications
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