Bethyl Laboratories
Rabbit anti-FOXP3 Recombinant Monoclonal Antibody [BLR034F]
Monoclonal Antibodies
Catalog #A700-034
TargetFOXP3
HostRabbit
CloneBLR034F
ReactivityHuman
ApplicationICC, IHC, IHC-IF, IP, mIF, WB
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Product Description
FOXP3 is a transcriptional regulator which is crucial for the development and inhibitory function of regulatory T-cells (Treg). It plays an essential role in maintaining homeostasis of the immune system by allowing the acquisition of full suppressive function and stability of the Treg lineage, and by directly modulating the expansion and function of conventional T-cells. FOXP3 can act either as a transcriptional repressor or a transcriptional activator depending on its interactions with other transcription factors, histone acetylases and deacetylases. [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q9BZS1].
Specifications
Clonality
Recombinant Monoclonal
Clone
BLR034F
Host
Rabbit
Immunogen
Between 275 and 325
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Purified
Concentration
1000 µg/ml
Target Identity
TargetFOXP3
Antigen Species
Human
Gene ID50943
Gene Symbol
FOXP3
Gene Name
forkhead box P3
Uniprot IDQ9BZS1
Protein Name
Forkhead box protein P3
Product Information
Applications
ICC, IHC, IHC-IF, IP, mIF, WB
Buffer
Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, BSA-Free
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Recombinant antibody was purified from cell culture supernatant.
Immunogen was a peptide representing a region between residue 275 and 325 of human Forkhead box P3 using the numbering given in entry NP_054728.2 (Gene ID 50943).
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunocytochemistry (ICC)1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
Immunohistochemistry (IHC)1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Immunohistochemistry-Immunofluoresence (IHC-IF)1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)20 µl/mg lysate
Multiplex Immunofluorescence (mIF)1:250
Western Blot (WB)1:1000
Documents
Product DatasheetPDF · A700-034 · Lot 2 (current lot)Open
Product DatasheetPDF · A700-034 · Lot 2 (current lot)Open
Safety Data SheetPDF · A700-034Open
Citations / Publications
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Resources
Standard Western Blot Protocol
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Immunoprecipitation Protocol
Read the Protocol
Simple Western™ Resources
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