Bethyl Laboratories
Rabbit anti-FOXP4 Antibody Affinity Purified
Primary
Catalog #A302-394A
TargetFOXP4
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP, WB
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Product Description
Forkhead box P4 (FOXP4) belongs to subfamily P of the forkhead box (FOX) transcription factor family. Forkhead box transcription factors play important roles in the regulation of tissue- and cell type-specific gene transcription during both development and adulthood. Many members of the forkhead box gene family, including members of subfamily P, have roles in mammalian oncogenesis. FOXP4 may play a role in the development of tumors of the kidney and larynx [taken from NCBI Entrez Gene (GeneID:116113)].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 1 and 50
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetFOXP4
Antigen Species
Human
Gene ID116113
Gene Symbol
FOXP4
Gene Name
forkhead box P4
Uniprot IDQ8IVH2
Protein Name
Forkhead box protein P4
Product Information
Applications
IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to FOXP4 immobilized on solid support.
The epitope recognized by A302-394A maps to a region between residue 1 and 50 of human forkhead box P4 using the numbering given in entry NP_001012426.1 (GeneID 116113).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:500 - 1:2500
Documents
Product DatasheetPDF · A302-394A · Lot 1 (current lot)Open
Product DatasheetPDF · A302-394A · Lot 1 (current lot)Open
Safety Data SheetPDF · A302-394AOpen
Citations / Publications
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