Bethyl Laboratories
Rabbit anti-FUS Antibody Affinity Purified
Primary
Catalog #A300-293A
TargetFUS
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIHC, IP, WB
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Product Description
FUS has been identified as a frequent translocation fusion partner of various transcription factors. FUS fusion genes have been shown to be associated with multiple tumor types which include liposarcoma, leukemia, histocytoma, and sarcoma. FUS is a multifunctional RNA-binding protein that associates with the nuclear matrix and Cajal bodies and appears to play a role in splicesome assembly, pre-mRNA splicing, DNA repair, transcriptional regulation and homologous recombination.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 450 and 500
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetFUS
Antigen Species
Human
Gene ID2521
Gene Symbol
FUS
Gene Name
FUS RNA binding protein
Uniprot IDP35637
Protein Name
RNA-binding protein FUS
Alternate Names
75 kDa DNA-pairing protein • ALS6 • ETM4 • FUS/ERG chimeric protein • FUS/ERG fusion protein • FUS1 • fused in sarcoma • fusion gene in myxoid liposarcoma • fus-like protein • heterogeneous nuclear ribonucleoprotein P2 • HNRNPP2 • oncogene FUS • oncogene TLS • POMP75 • RNA-binding protein FUS • TLS • translocated in liposarcoma protein
Product Information
Applications
IHC, IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to FUS immobilized on solid support.
The epitope recognized by A300-293A maps to a region between residues 400 and 450 of human fusion (involved in t(12;16) in malignant liposarcoma) using the numbering given in SwissProt entry P35637 (GeneID 2521).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:1000 to 1:5000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A300-293A · Lot 6 (current lot)Open
Product DatasheetPDF · A300-293A · Lot 6 (current lot)Open
Safety Data SheetPDF · A300-293AOpen
Citations / Publications
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