Bethyl Laboratories
Rabbit anti-G3BP1 Antibody Affinity Purified
Primary
Catalog #A302-034A
TargetG3BP1
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP, WB
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Product Description
Ras-GAP SH3 domain binding protein (G3BP1) contains an RNA recognition motif (RRM) and an N-terminal nuclear transport factor 2-like (NTF2-like) domain. It was first identified in a screen for proteins that bind the SH3 domain of the Ras GTPase activating protein (RasGAP). It has been shown to function downstream of Ras and play a role in RNA metabolism, signal transduction, and proliferation. G3BP1 is primarily cytoplasmic but has also been localized to the nucleus. G3BP1 has been shown to be overexpressed in human cancers.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 416 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetG3BP1
Antigen Species
Human
Gene ID10146
Gene Symbol
G3BP1
Gene Name
G3BP stress granule assembly factor 1
Uniprot IDQ13283
Protein Name
Ras GTPase-activating protein-binding protein 1
Alternate Names
ATP-dependent DNA helicase VIII • DNA helicase VIII • G3BP • G3BP-1 • GAP binding protein • GAP SH3 domain-binding protein 1 • GTPase activating protein (SH3 domain) binding protein 1 • hDH VIII • HDH-VIII • ras GTPase-activating protein-binding protein 1 • RasGAP-associated endoribonuclease G3BP • Ras-GTPase-activating protein SH3-domain-binding protein
Product Information
Applications
IP, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to G3BP1 immobilized on solid support.
The epitope recognized by A302-034A maps to a region between residue 416 and 466 of human GTPase activating protein (SH3 domain) binding protein 1 using the numbering given in entry NP_005745.1 (GeneID 10146).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A302-034A · Lot 1 (current lot)Open
Product DatasheetPDF · A302-034A · Lot 1 (current lot)Open
Product DatasheetPDF · A302-034A · Lot 1 (current lot)Open
Safety Data SheetPDF · A302-034AOpen
Citations / Publications
Services
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