Rabbit anti-G6PD Antibody Affinity Purified

Specifications

Production & Epitope

Antibody was affinity purified using an epitope specific to G6PD immobilized on solid support.

The epitope recognized by A300-404A maps to a region between residues 50 and 100 of human Glucose-6-Phosphate Dehydrogenase using the numbering given in entry NP_000393.2 (GeneID 2539).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).