Bethyl Laboratories
Rabbit anti-GAPDH Antibody Affinity Purified
Primary
Catalog #A300-643A
TargetGAPDH
HostRabbit
ClonalityPolyclonal
ReactivityMouse
ApplicationIHC, WB
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Product Description
In the process of carbohydrate metabolism, GAPDH (glyceraldehydes-3-phosphate dehydrogenase) catalyzes the oxidative phosphorylation of glyceraldehydes-3-phosphate to 1,3-diphosphoglycerate in the presence of nicotinamide adenine dinucleotide (NAD) and inorganic phosphate. In addition to its role in the oxidation of glucose, GAPDH has been shown to be involved in other cellular processes such as vesicle transport, DNA repair, DNA replication, cytoskeletal modulation, and tRNA export.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 283 and C-terminus
Isotype
IgG
Reactivity
Mouse
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetGAPDH
Antigen Species
Mouse
Gene ID14433
Gene Symbol
GAPDH
Gene Name
glyceraldehyde-3-phosphate dehydrogenase
Uniprot IDP16858
Protein Name
Glyceraldehyde-3-phosphate dehydrogenase
Alternate Names
Product Information
Applications
IHC, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to GAPDH immobilized on solid support.
The epitope recognized by A300-643A maps to a region between residues 300 and the C-terminus (residue 335) of mouse Glyceraldehyde-3-Phosphate Dehydrogenase using the numbering given in entry NP_032110.1 (GeneID 14433).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.78
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Immunohistochemistry (IHC)1:500 to 1:2000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A300-643A · Lot 3 (current lot)Open
Product DatasheetPDF · A300-643A · Lot 3 (current lot)Open
Safety Data SheetPDF · A300-643AOpen
Citations / Publications
Services
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