Bethyl Laboratories
Rabbit anti-GBF1 Antibody Affinity Purified
Primary
Catalog #A301-962A
TargetGBF1
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP
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Product Description
GBF1 is a guanine nucleotide exchange factor (GEF) that promotes the exchange of GDP for GTP on ADP ribosylation factor 5(ARF5). GBF1 activity is inhibited by brefeldin, an antibiotic that interferes with protein transport. It has been show to be important to golgi assembly and the coordination of bidirectional transport. Due to these characteristics, GBF1 is also called Golgi-specific brefeldin A-resistance GEF 1.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 650 and 700
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetGBF1
Antigen Species
Human
Gene ID8729
Gene Symbol
GBF1
Gene Name
golgi brefeldin A resistant guanine nucleotide exchange factor 1
Uniprot IDQ92538
Protein Name
Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1
Product Information
Applications
IP
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to GBF1 immobilized on solid support.
The epitope recognized by A301-962A maps to a region between residue 650 and 700 of human golgi-specific brefeldin A resistance factor 1 using the numbering given in entry NP_004184.1 (GeneID 8729).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Documents
Product DatasheetPDF · A301-962A · Lot 1 (current lot)Open
Product DatasheetPDF · A301-962A · Lot 1 (current lot)Open
Safety Data SheetPDF · A301-962AOpen
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