/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-GCF Antibody
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Primary Antibodies  

Rabbit anti-GCF Antibody Affinity Purified

bethyl
Bethyl Laboratories

Catalog #

Validated
Target:

GCF

Reactivity:

Human

,

Mouse

Applications:

IP

,

WB

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

between 175 and 225

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (200 µg/ml)

10 µl (200 µg/ml)

Product Details

Specifications
Verified Reactivity

Mouse,

Human

Antigen Species

Human

Concentration
200 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide


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Additional Product Information

GCF (GC-rich sequence DNA-binding factor) was identified as a factor that bound to GC-rich sequences and repressed transcription of the epidermal growth factor (EGF) receptor, beta-actin, and calcium-dependent protease promoters. Since its initial cloning and characterization, GCF has been observed to be a transcriptional repressor of several growth factor/receptor genes.

6936

GCFC2

GC-rich sequence DNA-binding factor 2

P16383

GC-rich sequence DNA-binding factor 2

Alternate Names

C2orf3; DNABF; GC binding factor; GC bindng factor; GCF; GC-rich sequence DNA-binding factor; GC-rich sequence DNA-binding factor 2; TCF9; TCF-9; Transcription factor 9; transcription factor 9 (binds GC-rich sequences)

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.