Bethyl Laboratories
Rabbit anti-GEF-H1 Antibody Affinity Purified
Primary
Catalog #A301-930A
TargetGEF-H1
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP
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Product Description
GEF-H1 (guanine nucleotide exchange factor H1) is a microtubule regulated guanine nucleotide exchange factor for the RhoA GTPase. It catalyzes the exchange of GDP for GTP by Rho in response to microtubule destabilization. GEF-H1 has shown to be regulated during cytokinesis and plays a role in the cleavage furrow.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 850 and 900
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetGEF-H1
Antigen Species
Human
Gene ID9181
Gene Symbol
ARHGEF2
Gene Name
Rho/Rac guanine nucleotide exchange factor 2
Uniprot IDQ92974
Protein Name
Rho guanine nucleotide exchange factor 2
Product Information
Applications
IP
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to GEF-H1 immobilized on solid support.
The epitope recognized by A301-930A maps to a region between residue 850 and 900 of human guanine nucleotide exchange factor H1 using the numbering given in entry CAH72629.1 (GeneID 9181).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Documents
Product DatasheetPDF · A301-930A · Lot 1 (current lot)Open
Product DatasheetPDF · A301-930A · Lot 1 (current lot)Open
Safety Data SheetPDF · A301-930AOpen
Citations / Publications
Services
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