Bethyl Laboratories
Rabbit anti-GFM1 Antibody Affinity Purified
Primary
Catalog #A305-114A
TargetGFM1
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationWB
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Product Description
The protein elongation factor G, mitochondrial, is encoded by the gene GFM1, also known as EGF1. This mitochondrial GTPase is involved in the polypeptide chain elongation pathway [taken from UniProtKB (Entry: Q96RP9)].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 1 and 50
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetGFM1
Antigen Species
Human
Gene ID85476
Gene Symbol
GFM1
Gene Name
G elongation factor mitochondrial 1
Uniprot IDQ96RP9
Protein Name
Elongation factor G, mitochondrial
Alternate Names
Product Information
Applications
WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to GFM1 immobilized on solid support.
The epitope recognized by A305-114A maps to a region between residue 25 to 75 of human Elongation factor G, mitochondrial using the numbering given in entry Q96RP9.2 (GeneID 85476).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western Blot (WB)1:1000 - 1:5000
Documents
Product DatasheetPDF · A305-114A · Lot 1 (current lot)Open
Product DatasheetPDF · A305-114A · Lot 1 (current lot)Open
Safety Data SheetPDF · A305-114AOpen
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