Bethyl Laboratories
Rabbit anti-GIT2 Antibody Affinity Purified
Primary
Catalog #A302-102A
TargetGIT2
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC, IP
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Product Description
G-protein-coupled receptor kinase interactor 1 (GIT1) and G-protein-coupled receptor kinase interactor 2 (GIT2) are GTPase-activating proteins for ADP-ribosylation (Arf) GTPases. GIT1 and GIT2 regulate Arf activity and the sequestration of activated G protein-coupled receptors from the surface of the cell. These activities have effects on multiple cellular processes such as cell migration, T-cell activation, and centrosome dynamics. Both GIT proteins also serve as scaffolds to link signaling molecules.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 500 and 550
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetGIT2
Antigen Species
Human
Gene ID9815
Gene Symbol
GIT2
Gene Name
GIT ArfGAP 2
Uniprot IDQ14161
Protein Name
ARF GTPase-activating protein GIT2
Alternate Names
ARF GAP GIT2 • ARF GTPase-activating protein GIT2 • CAT2 • CAT-2 • cool-associated, tyrosine phosphorylated protein 2 • cool-interacting tyrosine-phosphorylated protein 2 • G protein-coupled receptor kinase interacting ArfGAP 2 • G protein-coupled receptor kinase-interactor 2 • GRK-interacting protein 2 • Paxillin kinase linker • PKL
Product Information
Applications
IHC, IP
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to GIT2 immobilized on solid support.
The epitope recognized by A302-102A maps to a region between residue 500 and 550 of human G protein-coupled receptor kinase interacting ArfGAP 2 using the numbering given in entry NP_476510.1 (GeneID 9815).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:1000 - 1:5000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Documents
Product DatasheetPDF · A302-102A · Lot 1 (current lot)Open
Product DatasheetPDF · A302-102A · Lot 1 (current lot)Open
Safety Data SheetPDF · A302-102AOpen
Citations / Publications
Services
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