Bethyl Laboratories
Rabbit anti-GITR/TNFRSF18 Recombinant Monoclonal Antibody [BLR068G]
Monoclonal Antibodies
Catalog #A700-068
TargetGITR/TNFRSF18
HostRabbit
CloneBLR068G
ReactivityHuman
ApplicationFlow Cyt, ICC, IHC, IHC-IF, IP, WB
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Product Description
GITR/TNFRSF18 is a member of the TNF-receptor superfamily. It has been shown to have increased expression upon T-cell activation, and it is thought to play a key role in dominant immunological self-tolerance maintained by regulatory T cells. GITR/TNFRSF18 also plays a role in the regulation of CD3-driven T-cell activation and programmed cell death. [provided by RefSeq, Feb 2011] [taken from NCBI Entrez Gene (Gene ID: 8784)].
Specifications
Clonality
Recombinant Monoclonal
Clone
BLR068G
Host
Rabbit
Immunogen
Between 191 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Purified
Concentration
1000 µg/ml
Target Identity
TargetGITR/TNFRSF18
Antigen Species
Human
Gene ID8784
Gene Symbol
TNFRSF18
Gene Name
TNF receptor superfamily member 18
Uniprot IDQ9Y5U5
Protein Name
Tumor necrosis factor receptor superfamily member 18
Product Information
Applications
Flow Cyt, ICC, IHC, IHC-IF, IP, WB
Buffer
Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, BSA-Free
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Recombinant antibody was purified from cell culture supernatant.
Immunogen was a peptide representing a region between residue 191 and the C-terminus (residue 241) of human Tumor necrosis factor receptor superfamily member 18 (GITR) using the numbering given in entry NP_004186.1 (Gene ID 8784).
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Flow Cytometry (Flow Cyt)Fixed and permeabilized cells were stained with 0.2 µl per 1 x 10^6 cells.
Immunocytochemistry (ICC)1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
Immunohistochemistry (IHC)1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Immunohistochemistry-Immunofluoresence (IHC-IF)1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µl/mg lysate
Western Blot (WB)1:1000
Documents
Product DatasheetPDF · A700-068 · Lot 2 (current lot)Open
Product DatasheetPDF · A700-068 · Lot 2 (current lot)Open
Safety Data SheetPDF · A700-068Open
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