Rabbit anti-GLI1 Recombinant Monoclonal Antibody [BLR256L]
Product Details
Specifications
Request Formulation Change Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, BSA-Free
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Additional Product Information
GLI1, a member of the Kruppel family of zinc finger proteins, is a transcription factor activated by the sonic hedgehog signal transduction cascade that regulates stem cell proliferation. The activity and nuclear localization of GLI1 is negatively regulated by p53 in an inhibitory loop. [taken from NCBI Entrez Gene (Gene ID: 2735)].
Alternate Names
GLI; GLI-Kruppel family member GLI1; Glioma-associated oncogene; glioma-associated oncogene 1; glioma-associated oncogene homolog 1 (zinc finger protein); oncogene GLI; PAPA8; zinc finger protein GLI1
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.