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Primary Antibodies  

Rabbit anti-GMDS Antibody Affinity Purified

Bethyl Laboratories®
Bethyl Laboratories®

Catalog #

Validated
Target:

GMDS

Reactivity:

Human

Applications:

IP

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

Between 322 and 372

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

10 µl (1000 µg/ml)

100 µl (1000 µg/ml)

Product Details

Specifications
Verified Reactivity

Human

Presumed Reactivity

Mouse

Antigen Species

Human

Concentration
1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide


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Additional Product Information

GDP-mannose 4,6-dehydratase (GMDS) catalyzes the conversion of GDP-mannose to GDP-4-keto-6-deoxymannose, the first step in the synthesis of GDP-fucose from GDP-mannose, using NADP+ as a cofactor. The second and third steps of the pathway are catalyzed by a single enzyme, GDP-keto-6-deoxymannose 3,5-epimerase, 4-reductase, designated FX in humans (MIM 137020) [taken from NCBI Entrez Gene (Gene ID: 2762)].

2762

GMDS

GDP-mannose 4,6-dehydratase

O60547

GDP-mannose 4,6 dehydratase

Alternate Names

GDP-D-mannose dehydratase; GDP-mannose 4,6 dehydratase; GMD; SDR3E1; short chain dehydrogenase/reductase family 3E, member 1

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.