Bethyl Laboratories

Rabbit anti-GNL1 Antibody Affinity Purified

Primary
Catalog #A302-246A
TargetGNL1
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP
Detection of human GNL1 by western blot of immunoprecipitates.
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Product Description

GNL1 (guanine nucleotide-binding protein-like 1) is a GTP-binding protein. Its gene has been found to map to the human major histocompatibility complex class I region indicating that it may have a role in the signal transduction of T-cell mediated immunity.

Specifications

Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 1 and 50
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetGNL1
Antigen Species
Human
Gene ID2794
Gene Symbol
GNL1
Gene Name
G protein nucleolar 1 (putative)
Uniprot IDP36915
Protein Name
Guanine nucleotide-binding protein-like 1
Product Information
Applications
IP
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA

Production & Epitope

Antibody was affinity purified using an epitope specific to GNL1 immobilized on solid support.

The epitope recognized by A302-246A maps to a region between residue 1 and 50 of human guanine nucleotide binding protein-like 1 using the numbering given in entry NP_005266.2 (GeneID 2794).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.

Immunoprecipitation (IP)6 µg/mg lysate

Documents

Product DatasheetPDF · A302-246A · Lot 1 (current lot)Open
Product DatasheetPDF · A302-246A · Lot 1 (current lot)Open
Safety Data SheetPDF · A302-246AOpen
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