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Rabbit anti-GNL2 Antibody Affinity Purified

bethyl
Bethyl Laboratories Catalog #
Validated
Target:
GNL2
Reactivity:
Human, Mouse
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
Between 681 and C-terminus
Isotype:
IgG
Conjugate:
Unconjugated
Purity:
Antigen Affinity Purified
100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

$131.00 $464.00
Qty:

Product Details

Specifications

Verified Reactivity
Mouse, Human
Antigen Species
Human
Concentration
1000 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
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Production Procedures
Antibody was affinity purified using an epitope specific to GNL2 immobilized on solid support.

The epitope recognized by A305-155A maps to a region between residue 0 of human Nucleolar GTP-binding protein 2 using the numbering given in entry Q13823.1 (GeneID 29889).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Antibody was affinity purified using an epitope specific to GNL2 immobilized on solid support.

The epitope recognized by A305-155A-T maps to a region between residue 0 of human Nucleolar GTP-binding protein 2 using the numbering given in entry Q13823.1 (GeneID 29889).
Country of Origin
USA

Additional Product Information

Nucleolar GTP-binding protein 2, encoded by the gene GNL2, is a GTPase that associates with pre-60S ribosomal subunits in the nucleolus and is required for their nuclear export and maturation [taken from UniProtKB (Entry: Q13823)].

29889
GNL2
G protein nucleolar 2
Q13823
Nucleolar GTP-binding protein 2

Alternate Names

autoantigen NGP-1; guanine nucleotide binding protein-like 2 (nucleolar); HUMAUANTIG; NGP1; Ngp-1; Nog2; novel nucleolar guanosine 5'-triphosphate binding protein autoantigen; nucleolar G-protein gene 1; nucleolar GTPase; nucleolar GTP-binding protein 2; nucleostemin-2; Nug2

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.