Bethyl Laboratories
Rabbit anti-GRSF1 Antibody Affinity Purified
Primary
Catalog #A305-137A
TargetGRSF1
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP, WB
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Product Description
The protein G-rich sequence factor 1 is encoded by the gene GRSF1. G-rich sequence factor 1 is a cellular protein that binds RNAs containing the G-rich element. The protein is localized in the cytoplasm, and has been shown to stimulate translation of viral mRNAs in vitro [taken from NCBI Entrez Gene (Gene ID: 2926)].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 325 and 375
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetGRSF1
Antigen Species
Human
Gene ID2926
Gene Symbol
GRSF1
Gene Name
G-rich RNA sequence binding factor 1
Uniprot IDQ12849
Protein Name
G-rich sequence factor 1
Alternate Names
Product Information
Applications
IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to GRSF1 immobilized on solid support.
The epitope recognized by A305-137A maps to a region between residue 325 to 375 of human G-rich sequence factor 1 using the numbering given in entry Q12849.3 (GeneID 2926).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A305-137A · Lot 1 (current lot)Open
Product DatasheetPDF · A305-137A · Lot 1 (current lot)Open
Safety Data SheetPDF · A305-137AOpen
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