Bethyl Laboratories
Rabbit anti-GRWD1/WDR28 Antibody Affinity Purified
Primary
Catalog #A301-577A
TargetGRWD1/WDR28
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP, WB
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Product Description
GRWD1/WDR28 contains five WD repeats and is a member of the WD family of proteins. The WD repeat is defined by four or more repeating units of a conserved core of approximately 40 amino acids ending with tryptophan-aspartic acid (WD). WD repeats may serve as sites of protein-protein interaction for adaptor proteins and facilitate multiprotein complex formation. WD proteins are involved in a variety of cellular processes. GRWD1/WDR28 is proposed to play a role in ribosome biogenesis.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 375 and 425
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetGRWD1/WDR28
Antigen Species
Human
Gene ID83743
Gene Symbol
GRWD1
Gene Name
glutamate rich WD repeat containing 1
Uniprot IDQ9BQ67
Protein Name
Glutamate-rich WD repeat-containing protein 1
Product Information
Applications
IP, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to GRWD1/WDR28 immobilized on solid support.
The epitope recognized by A301-577A maps to a region between residue 350 and 400 of human glutamate-rich WD repeat containing 1 (WD repeat domain 28) using the numbering given in entry NP_113673.2 (GeneID 83743).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A301-577A · Lot 1 (current lot)Open
Product DatasheetPDF · A301-577A · Lot 1 (current lot)Open
Safety Data SheetPDF · A301-577AOpen
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