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Rabbit anti-GST-Tag Recombinant Monoclonal Antibody [BLRE05M]

bethyl
Bethyl Laboratories

Catalog # A191-105

Validated
Target:

GST-Tag

Host:

Rabbit

Clonality:

Recombinant Monoclonal

Clone:

BLRE05M

Format:

Whole IgG

Immunogen:

residues 1-201

Isotype:

IgG

Purity:

Purified

100 µl (10 blots)

A191-105
$482.00
Qty:

Product Details

Specifications
Concentration
40 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Phosphate Buffered Saline (PBS) with 0.1% BSA and 0.09% Sodium Azide


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Production Procedures

Recombinant antibody was purified from cell culture supernatant.

Immunogen was a recombinant protein representing Escherichia coli Glutathione S-transferase GstA (residues 1-201, WP_000765749.1).

Country of Origin

USA

Additional Product Information

GST (glutathione S-transferase) is commonly engineered onto the N- or C- terminus of a protein of interest to create fusion proteins that can be purified, visualized and analyzed via the properties of the GST tag.

945758

gstA

glutathione S-transferase GstA

P0A9D2

Glutathione S-transferase GstA

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (A120-113P) with 5% Normal Pig Serum (S100-020) added to the blocking buffer.