Bethyl Laboratories
Rabbit anti-HCA66 Antibody Affinity Purified
Primary
Catalog #A302-574A
TargetHCA66
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP
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Product Description
HCA66 (hepatocellular carcinoma-associated antigen 66) is also known as UTP6 and is a subunit of the UtpB subcomplex which is part of the SSU (small subunit) processome. HCA66 contains a HAT (half-a-tetracopeptide repeat) domain which is found only in proteins involved in RNA metabolism. HCA66 has also been identified as a component of the centrosome and as a regulator of gamma-tubulin.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 547 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetHCA66
Antigen Species
Human
Gene ID55813
Gene Symbol
UTP6
Gene Name
UTP6, small subunit processome component
Uniprot IDQ9NYH9
Protein Name
U3 small nucleolar RNA-associated protein 6 homolog
Product Information
Applications
IP
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to HCA66 immobilized on solid support.
The epitope recognized by A302-574A maps to a region between residue 530 and 565 of human hepatocellular carcinoma associated antigen 66 using the numbering given in entry NP_060898.2 (GeneID 55813).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Documents
Product DatasheetPDF · A302-574A · Lot 1 (current lot)Open
Product DatasheetPDF · A302-574A · Lot 1 (current lot)Open
Safety Data SheetPDF · A302-574AOpen
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