/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-HCF1 Antibody
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Primary Antibodies  

Rabbit anti-HCF1 Antibody Affinity Purified

bethyl
Bethyl Laboratories

Catalog #

Validated
Citations (22)
Target:

HCF1

Reactivity:

Human

Applications:

IP

,

WB

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

Between 1985 and C-terminus

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (200 µg/ml)

10 µl (200 µg/ml)

$121.00 $433.00
Qty:
Bulk Request

Product Details

Specifications
Verified Reactivity

Human

Presumed Reactivity

Mouse,

Golden Hamster

Antigen Species

Human

Concentration
200 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide


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Production Procedures

Antibody was affinity purified using an epitope specific to HCF1 immobilized on solid support.

The epitope recognized by A301-400A maps to a region between residue 1985 and 2035 of human host cell factor C1 (VP16-accessory protein) using the numbering given in entry NP_005325.2 (GeneID 3054).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Antibody was affinity purified using an epitope specific to HCF1 immobilized on solid support.

The epitope recognized by A301-400A-T maps to a region between residue 1985 and 2035 of human host cell factor C1 (VP16-accessory protein) using the numbering given in entry NP_005325.2 (GeneID 3054).

Country of Origin

USA

Additional Product Information

HCF1 was originally identified as a host-cell factor utilized by the herpes simplex virus to promote viral transcription. In the cell, HCF1 functions as a transcriptional co-regulator involved in the modification of histones and cell cycle progression.

3054

HCFC1

host cell factor C1

P51610

Host cell factor 1

Alternate Names

C1 factor; CFF; HCF; HCF1; HCF-1; HFC1; host cell factor 1; MRX3; PPP1R89; protein phosphatase 1, regulatory subunit 89; VCAF; VP16 accessory protein; VP16-accessory protein

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.