Bethyl Laboratories
Rabbit anti-HDAC7 Antibody Affinity Purified
Primary
Catalog #A301-384A
TargetHDAC7
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC, WB
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Product Description
Histone deacetylase 7 (HDAC7) is a class II histone deacetylase that catalyzes the removal of acetyl groups from core histone octamer subunits. HDAC7 appears to have an influence on cellular processes by acting as a transcriptional repressor. HDAC7 is highly expressed in double positive thymocytes and may play an important role in T-cell development. Recently, HDAC7 has also been reported to influence endothelial cell migration and modulate angiogenesis.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 425 and 475
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetHDAC7
Antigen Species
Human
Gene ID51564
Gene Symbol
HDAC7
Gene Name
histone deacetylase 7
Uniprot IDQ8WUI4
Protein Name
Histone deacetylase 7
Alternate Names
Product Information
Applications
IHC, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to HDAC7 immobilized on solid support.
The epitope recognized by A301-384A maps to a region between residue 425 and 475 of human histone deacetylase 7A using the numbering given in entry NP_056216.1 (GeneID 51564).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Immunohistochemistry (IHC)1:500 - 1:2000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A301-384A · Lot 1 (current lot)Open
Product DatasheetPDF · A301-384A · Lot 1 (current lot)Open
Safety Data SheetPDF · A301-384AOpen
Citations / Publications
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