Bethyl Laboratories
Rabbit anti-HECTD1 Antibody Affinity Purified
Primary
Catalog #A302-908A
TargetHECTD1
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP, WB
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Product Description
HECT domain-containing protein 1 (HECTD1) is a probable E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. HECTD1 may be required for development of the head mesenchyme and neural tube closure [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/ Q9ULT8].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 1 and 50
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetHECTD1
Antigen Species
Human
Gene ID25831
Gene Symbol
HECTD1
Gene Name
HECT domain E3 ubiquitin protein ligase 1
Uniprot IDQ9ULT8
Protein Name
E3 ubiquitin-protein ligase HECTD1
Product Information
Applications
IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to HECTD1 immobilized on solid support.
The epitope recognized by A302-908A maps to a region between residue 1 and 50 of human HECT Domain Containing 1 using the numbering given in entry AAP13073.1 (GeneID 25831).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000 (3-8% Tris-acetate gel recommended)
Documents
Product DatasheetPDF · A302-908A · Lot 1 (current lot)Open
Product DatasheetPDF · A302-908A · Lot 1 (current lot)Open
Safety Data SheetPDF · A302-908AOpen
Citations / Publications
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