Rabbit anti-HEXIM2 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A303-077A-T maps to a region between residue 1 and 50 of human Hexamthylene Bis-Acetamide Inducible 2 using the numbering given in entry NP_653209.1 (GeneID 124790).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to HEXIM2 immobilized on solid support.
The epitope recognized by A303-077A maps to a region between residue 1 and 50 of human Hexamthylene Bis-Acetamide Inducible 2 using the numbering given in entry NP_653209.1 (GeneID 124790).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
HEXIM2 (Hexamethylene bis-acetamide-inducible protein 2) is a transcriptional regulator which functions as a general RNA polymerase II transcription inhibitor. In cooperation with 7SK snRNA, HEXIM2 sequesters P-TEFb in a large inactive 7SK snRNP complex preventing RNA polymerase II phosphorylation and subsequent transcriptional elongation. [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/ Q96MH2].
Alternate Names
hexamethylene bis-acetamide inducible 2; hexamethylene bis-acetamide-inducible protein 2; hexamethylene-bis-acetamide-inducible transcript 2; L3; MAQ1 paralog; protein HEXIM2
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.