Rabbit anti-HIF1-alpha Antibody Affinity Purified

Specifications

Production & Epitope

Antibody was affinity purified using an epitope specific to HIF1-alpha immobilized on solid support.

The epitope recognized by A300-286A maps to a region between residues 775 and the C-terminus (residue 826) of human hypoxia-inducible factor 1, alpha subunit using the numbering given in Swiss-Prot entry Q16665 (GeneID 3091).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).

Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.