Bethyl Laboratories
Rabbit anti-HIF1-alpha IHC Antibody Affinity Purified
Primary
Catalog #IHC-00460
TargetHIF1-alpha
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationICC-IF, IHC
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Product Description
Hypoxia-inducible factor-1 (HIF1) is a basic helix-loop-helix (bHLH)-PER-ARNT-sim (PAS) transcription factor that plays a role in the cellular response to hypoxia. HIF1 is a heterodimer of an HIF1-alpha and HIF1-beta subunit. During normoxia, HIF-1alpha is regulated by ubiquitination and interaction with the von-Hippel-Lindau tumor suppressor protein (pVHL).
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 776 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetHIF1-alpha
Antigen Species
Human
Gene ID3091
Gene Symbol
HIF1A
Gene Name
hypoxia inducible factor 1 subunit alpha
Uniprot IDQ16665
Protein Name
Hypoxia-inducible factor 1-alpha
Alternate Names
ARNT-interacting protein • basic-helix-loop-helix-PAS protein MOP1 • bHLHe78 • class E basic helix-loop-helix protein 78 • HIF1 • HIF-1A • HIF-1alpha • HIF-1-alpha • HIF1-ALPHA • hypoxia inducible factor 1 alpha subunit • hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) • hypoxia-inducible factor 1 alpha isoform I.3 • hypoxia-inducible factor 1-alpha • hypoxia-inducible factor1alpha • member of PAS protein 1 • member of PAS superfamily 1 • MOP1 • PAS domain-containing protein 8 • PASD8
Product Information
Applications
ICC-IF, IHC
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to HIF1-alpha immobilized on solid support.
The epitope recognized by IHC-00460 maps to a region between residues 775 and the C-terminus (residue 826) of human hypoxia-inducible factor 1, alpha subunit using the numbering given in Swiss-Prot entry Q16665 (GeneID 3091).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
Epitope exposure is recommended.
Epitope exposure with tris-EDTA pH9.0 buffer will enhance staining.
Likely to work with frozen sections.
In some cases, the antibody may be diluted further than indicated.
Immunohistochemistry (IHC)1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 for 20 minutes using a pressure cooker is recommended for FFPE tissue sections.
Immunocytochemistry-Immunofluoresence (ICC-IF)1:100 - 1:500
Documents
Safety Data SheetPDF · IHC-00460Open
Product DatasheetPDF · IHC-00460 · Lot 4 (current lot)Open
Product DatasheetPDF · IHC-00460 · Lot 4 (current lot)Open
Citations / Publications
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