/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-HIF2-alpha Recombinant Monoclonal Antibody [BL-95-1A2]
NEW!
Primary Antibodies  

Rabbit anti-HIF2-alpha Recombinant Monoclonal Antibody [BL-95-1A2]

bethyl
Bethyl Laboratories

Catalog #

Validated
Citations (19)
Target:

HIF2-alpha

Reactivity:

Human

Applications:

ChIP

,

ICC

,

ICC-IF

,

IHC

,

IP

,

WB

Host:

Rabbit

Clonality:

Recombinant Monoclonal

Clone:

BL-95-1A2

Format:

Whole IgG

Immunogen:

Between 400 and 450

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Purified

100 µl (10 blots)

20 µl (2 blots)

100 µg (BSA-Free)

$127.00 $794.00
Qty:
Bulk Request

Product Details

Specifications
Verified Reactivity

Human

Antigen Species

Human

Concentration
400 µg/ml 1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide


Request Formulation Change

Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, BSA-Free


Request Formulation Change
Production Procedures

Recombinant antibody was purified from cell culture supernatant.

The epitope recognized by A700-003-T maps to a region between residues 400 and 450 of human endothelial PAS domain protein 1 using the numbering given in entry NP_001421.2 (GeneID 2034).

Recombinant antibody was purified from cell culture supernatant.

The epitope recognized by A700-003 maps to a region between residues 400 and 450 of human endothelial PAS domain protein 1 using the numbering given in entry NP_001421.2 (GeneID 2034).

Country of Origin

USA

Additional Product Information

The gene EPAS1 encodes for the transcription factor endothelial PAS domain-containing protein 1, and is induced as oxygen levels fall. Commonly called hypoxia inducible factor 2A (HIF2a), this protein is involved in the induction of oxygen regulated genes, binding to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. An important cancer biomarker, HIF2a may also play a role in Tibetan high-altitude adaptation (Am J Hum Genet. 2015 Jul 2; 97(1): 54–66.) and mutations in the EPAS1 gene are associated with erythrocytosis familial type 4 [taken from NCBI Entrez Gene (Gene ID: 2034) and UniProtKB (Entry: Q99814)].

2034

EPAS1

endothelial PAS domain protein 1

Q99814

Endothelial PAS domain-containing protein 1

Alternate Names

basic-helix-loop-helix-PAS protein MOP2; bHLHe73; class E basic helix-loop-helix protein 73; ECYT4; endothelial PAS domain-containing protein 1; EPAS-1; HIF-1-alpha-like factor; HIF-1alpha-like factor; HIF2A; HIF-2-alpha; HIF2-alpha; HLF; hypoxia-inducible factor 2 alpha; Hypoxia-inducible factor 2-alpha; member of PAS protein 2; MOP2; PAS domain-containing protein 2; PASD2

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.