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Rabbit anti-HINT1 Antibody

Bethyl Laboratories®

Bethyl Laboratories® Catalog #

Validated
Target:
HINT1
Reactivity:
Human
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
Between 1 and 50
IsoType:
IgG
Purity:
Antigen Affinity Purified
100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

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Product Details

Specifications
Verified Reactivity
Human
Presumed Reactivity
Rabbit, Bovine, Orangutan, Rat, Mouse
Antigen Species
Human
Stock Concentration
1000 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
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Prod Procedures
Antibody was affinity purified using an epitope specific to HINT1 immobilized on solid support.

The epitope recognized by A305-363A-T maps to a region between residue 1 to 50 of human Histidine triad nucleotide-binding protein 1 using the numbering given in entry P49773.2 (GeneID 3094).

Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

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Gene & Protein Information

Histidine triad nucleotide-binding protein 1 (HINT1) hydrolyzes purine nucleotide phosphoramidates substrates, including AMP-morpholidate, AMP-N-alanine methyl ester, AMP-alpha-acetyl lysine methyl ester, and AMP-NH2. The encoded protein interacts with these substrates via a histidine triad motif [taken from NCBI Entrez Gene (Gene ID: 3094)].

3094

HINT1

histidine triad nucleotide binding protein 1

P49773

Histidine triad nucleotide-binding protein 1

Alternate Names

adenosine 5'-monophosphoramidase, epididymis secretory sperm binding protein, HINT, histidine triad nucleotide-binding protein 1, NMAN, PKCI-1, PRKCNH1, protein kinase C inhibitor 1, protein kinase C-interacting protein 1

Applications

Western blot of immunoprecipitates performed using Normal Pig Serum (Cat. No. S100-020), Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) and 4-12% SDS-PAGE.