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Rabbit anti-HMCES Antibody Affinity Purified

bethyl
Bethyl Laboratories Catalog #
Validated
Target:
HMCES
Reactivity:
Human
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
Between 304 and C-terminus
Isotype:
IgG
Conjugate:
Unconjugated
Purity:
Antigen Affinity Purified
100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

$121.00 $433.00
Qty:

Product Details

Specifications

Verified Reactivity
Human
Antigen Species
Human
Concentration
1000 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
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Country of Origin
USA

Additional Product Information

HMCES specifically binds 5-hydroxymethylcytosine (5hmC)-containing DNA in stem cells, suggesting that it acts as a specific reader of 5hmC in stem cells (By similarity) [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q96FZ2].

56941
HMCES
5-hydroxymethylcytosine binding, ES cell specific
Q96FZ2
Embryonic stem cell-specific 5-hydroxymethylcytosine-binding protein

Alternate Names

5-hydroxymethylcytosine (hmC) binding, ES cell-specific; C3orf37; DC12; embryonic stem cell-specific 5-hydroxymethylcytosine-binding protein; ES cell-specific 5hmC-binding protein; putative peptidase SRAPD1; SOS response associated peptidase domain containing 1; SRAP domain-containing protein 1; SRAPD1; UPF0361 protein C3orf37

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.